PhD in Biochemical characterisation of signal integration in plants

Original title: PhD position - Biochemical characterisation of signal integration in plants

• identify candidate B4-RAF signalosome components through immunoprecipitation coupled to tandem mass spectrometry (IP–MS/MS), building on existing proximity-labelling datasets;
• validate protein interactions and co-localisation using approaches such as FRET-FLIM, co-immunoprecipitation and live-cell confocal imaging;
• generate and characterise Arabidopsis loss-of-function and fluorescent reporter lines;
• use microfluidics-coupled live-cell imaging to investigate the spatiotemporal dynamics, environmental conditionality and subcellular localisation of B4-RAF signalling complexes;
• integrate proteomics, imaging and genetic datasets to develop mechanistic models of rapid plant signal integration.
• complete a PhD thesis within the official appointment duration (four years);
• systematically and independently design, perform, analyse and document experiments;
• be an active and responsible member of the research group
• collaborate closely with other group members (incl. PhD candidate, technicians and postdoc);
• discuss work with group members and at departmental meetings, and incorporate feedback;
• take a leading role in writing manuscripts for publication in peer-reviewed journals;
• participate in the PhD training programme of the University of Amsterdam and the national graduate school Experimental Plant Sciences (EPS);
• assist with teaching and supervision of Bachelor’s and Master’s students.
• work at the interface of plant genetics, biochemistry, functional proteomics and quantitative imaging;
• present results at national and international scientific conferences;
• expand academic, professional and personal skills;
• contribute to science communication and outreach activities;
• thrive in a team that values impactful research and mutual support;
• combine the advantages that a collaborative project offers, including access to state-of-the-art research facilities at the Swammerdam Institute and the University of Amsterdam.
• an MSc degree in molecular biology, biochemistry, or a closely related discipline.
• demonstrated hands-on laboratory experience in at least two of the following areas: protein biochemistry, (plant) molecular biology, confocal microscopy, proteomics and protein-protein interaction assays;
• ability to plan experiments carefully, maintain high-quality laboratory records, analyse results critically and troubleshoot experimental work;
• fluent in English, both written and spoken;
• ability to work independently as well as constructively in an international team.
• 232 holiday hours per year (based on fulltime);
• multiple courses to follow from our Teaching and Learning Centre;
• a complete educational program for PhD students;
• a pension at ABP for which UvA pays two third part of the contribution;
• the possibility to follow courses to learn Dutch;
• help with housing for a studio or small apartment when you’re moving from abroad.
• a detailed CV including the months (not just years) when referring to your education and work experience;
• a letter of motivation;
• the names and email addresses of two references who can provide letters of recommendation.