PhD position - Biochemical characterisation of signal integration in plants

Official university vacancy imported from University of Amsterdam. Details are presented for discovery and may change. Confirm the latest requirements and apply on the official source.

• identify candidate B4-RAF signalosome components through immunoprecipitation coupled to tandem mass spectrometry (IP–MS/MS), building on existing proximity-labelling datasets;
• validate protein interactions and co-localisation using approaches such as FRET-FLIM, co-immunoprecipitation and live-cell confocal imaging;
• generate and characterise Arabidopsis loss-of-function and fluorescent reporter lines;
• use microfluidics-coupled live-cell imaging to investigate the spatiotemporal dynamics, environmental conditionality and subcellular localisation of B4-RAF signalling complexes;
• integrate proteomics, imaging and genetic datasets to develop mechanistic models of rapid plant signal integration.
• complete a PhD thesis within the official appointment duration (four years);
• systematically and independently design, perform, analyse and document experiments;
• be an active and responsible member of the research group
• collaborate closely with other group members (incl. PhD candidate, technicians and postdoc);
• discuss work with group members and at departmental meetings, and incorporate feedback;
• take a leading role in writing manuscripts for publication in peer-reviewed journals;
• participate in the PhD training programme of the University of Amsterdam and the national graduate school Experimental Plant Sciences (EPS);
• assist with teaching and supervision of Bachelor’s and Master’s students.
• work at the interface of plant genetics, biochemistry, functional proteomics and quantitative imaging;
• present results at national and international scientific conferences;
• expand academic, professional and personal skills;
• contribute to science communication and outreach activities;
• thrive in a team that values impactful research and mutual support;
• combine the advantages that a collaborative project offers, including access to state-of-the-art research facilities at the Swammerdam Institute and the University of Amsterdam.
• an MSc degree in molecular biology, biochemistry, or a closely related discipline.
• demonstrated hands-on laboratory experience in at least two of the following areas: protein biochemistry, (plant) molecular biology, confocal microscopy, proteomics and protein-protein interaction assays;
• ability to plan experiments carefully, maintain high-quality laboratory records, analyse results critically and troubleshoot experimental work;
• fluent in English, both written and spoken;
• ability to work independently as well as constructively in an international team.
• 232 holiday hours per year (based on fulltime);
• multiple courses to follow from our Teaching and Learning Centre;
• a complete educational program for PhD students;
• a pension at ABP for which UvA pays two third part of the contribution;
• the possibility to follow courses to learn Dutch;
• help with housing for a studio or small apartment when you’re moving from abroad.
• a detailed CV including the months (not just years) when referring to your education and work experience;
• a letter of motivation;
• the names and email addresses of two references who can provide letters of recommendation.